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Last pushed: 15 days ago
Short Description
Tools suite for simple eukaryotics genomes expert annotation using proteomics technologies
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From the use of MS/MS analysis data, the software has to : 1) confirm genes predictions ; 2) annotate new CDS; 3) correct intron-exon junctions. First, Peptide Sequence Tags (PST) are generated from MS/MS spectra analysis using the software Pepnovotag 3.1 beta [3]. A PST is defined as a short peptide sequence (3 to 5 amino acids) flanked by two masses corresponding to the two adjacent polypeptides. We align and clusterize PSTs in the six reading frames using two modules of PepLine [3] (PMmatch and PMclust) adapted and optimized for the project. So the clusters obtained are evaluated and compared with the genes predictions produced by the conventional genome annotation.

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