alex1075/flimkit repository overview

⁠FLIMKit⁠

FLIMKit is a Python toolkit for FLIM data from Leica SP8/FALCON (or any PTU-based system). Built as a drop-in for Leica LAS X FLIM analysis, with two workflows:

• Reconvolution fitting: mono/bi/tri-exponential lifetime fitting with full IRF deconvolution, per-pixel and summed modes, multi-tile ROI stitching, and batch processing
• Phasor analysis: calibrated phasor plots, interactive elliptical cursors, spatial filtering (gaussian/median/wavelet), two-component decomposition, automatic peak detection, session save/load

Four entry points: desktop GUI, guided terminal UI, CLI scripts, Python API.

FLIMKit github repo⁠ | Full documentation⁠

⁠Outputs

⁠Roadmap

Done: single FOV fitting, tile stitching, batch ROI processing, phasor analysis, GUI, session restoration, compiled app, ROI analysis with QuPath export.

Up next: config persistence, stat histograms, auto-region detection, batch n-exp in GUI. Chemical validation and publication pending.

⁠Contact

Alex Hunt: [email protected]⁠