fortin946/crisprverse repository overview

⁠Description

Docker image for the crisprVerse ecosystem⁠ using R 4.2.1. Dockerfile used to create the image can be found here⁠. The container contains the following components:

• R 4.2.1
• crisprVerse R packages and their dependencies
• ViennaRNA 2.5.1
• RNAhybrid 2.1.2

⁠About the crisprVerse

The crisprVerse is a Bioconductor ecosystem of R packages that enables efficient gRNA design and annotation for a multitude of CRISPR technologies, including CRISPR knockout (CRISPRko), CRISPR activation (CRISPRa), CRISPR interference (CRISPRi), CRISPR base editing (CRISPRbe), and CRISPR knockdown (CRISPRkd). The core package, crisprDesign, offers a comprehensive, user-friendly, and unified interface to add on- and off-target annotations via several alignment methods, rich gene and SNP annotations, and a dozen on- and off-target activity scores. These functionalities are enabled for any RNA- or DNA-targeting nucleases, including Cas9, Cas12, and Cas13. Both single and paired gRNA designs are enabled.

⁠Authors

Jean-Philippe Fortin, Pirunthan Perampalam, Aaron Lun, Luke Hoberecht

⁠License

Copyright Genentech Inc.