leilaicruz/satay repository overview

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SATAY and Transposonmapper

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This workflow is created for processing sequencing data for SAturated Transposon Analysis in Yeast (SATAY) for Saccharomyces Cerevisiae. It performs the steps from raw sequencing data until the transposon mapping that outputs files containing all insertion sites combined with the number of reads.

For more information regarding SATAY, see https://sites.google.com/site/satayusers/⁠_ created by the Kornmann-lab. For more extensive documentation, see our JupyterBook , https://satay-ll.github.io/SATAY-jupyter-book/Introduction.html⁠ .

The workflow requires input sequencing data in fastq format. It can perform the following tasks:

• sequence trimming
• quality checking raw and trimmed fastq files
• sequence alignment with reference genome (S288C Cerevisiae genome)
• quality checking bam files, indexing and sorting
• transposon mapping

The output files indicate the location of transposon insertions and the number of reads at those locations. This is presented in both .bed and .wig format. Also a list of genes is generated where the number and distribution of insertions and reads is presented per (essential) gene.