thebiohub/microbiome repository overview

⁠16S rRNA Amplicon Sequencing Analysis Pipeline

A containerized workflow for microbiome analysis of 16S rRNA amplicon sequencing data, built for reproducible and scalable processing from raw reads to community-level insights.

⁠Features

• DADA2 — high-resolution amplicon sequence variant (ASV) inference
• Primer trimming and quality filtering
• Taxonomic classification against reference databases (e.g., SILVA, GTDB)
• Alpha and beta diversity analyses
• Publication-ready visualizations

⁠Usage

docker pull thebiohub/microbiome

⁠Requirements

• Docker 20.10+
• Minimum 8GB RAM recommended

⁠Citation

If you use this workflow in your research, please cite the relevant tools:

• Callahan BJ et al. (2016) DADA2. Nature Methods. doi:10.1038/nmeth.3869

⁠Maintained by

TheBioHub⁠